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质谱学报
第 30 卷 增刊
磷酰化肽酯及小肽与氨基酸相互作用的 ESI-MS 研究
强黎明 1,2 ,杨 柳 1,曹书霞 2,卢 奎 1,赵玉芬 2
(1. 河南工程学院材料与化学工程系,河南 郑州 450007; 2. 郑州大学化学系,河南省化学生物学与有机化学重点实验室,河南 郑州 450052)
Study on the Interaction between Phosphoryl Dipeptides (or Methyl Esters) and Amino Acids by ESI-MS
QIANG Li-ming1,2, YANG Liu1, CAO Shu-xia2, LU Kui1, ZHAO Yu-fen2
(1. Department of Material and Chemical Engineering, Henan Institute of Engineering, Zhengzhou 450007, China; 2. Henan Province Key Laboratory of Chemical Biology and Organic Chemistry, Department of Chemistry, Zhengzhou University, Zhengzhou 450052, China) Abstract: The non-covalent interaction between a series of N-diisopropylphosphoryl dipeptides(or methyl esters) (DPP) and amino acids was studied by ESI-MS. The results show that aromatic rings to form π–π conjugated system could greatly strengthen the interaction. Also, the polar groups generating hydrogen bonds are favourable to the complexes. In addition, the molecule size, flexibility and steric hindrance show obvious effects on the interaction. While, the chirality of amino acids has no effect on the interaction between DPP and amino acids under ESI-MS conditions. Key words: ESI-MS; N-phosphoryl dipeptides(or methyl esters); amino acids; non-covalent weak interaction 中图分类号:O 657.63 文献标识码:A 文章编号:1004-2997(2009)增刊-0024-02
目前, 电喷雾质谱 (ESI-MS) 已被证明是一种检测, 分析分子间非共价相互作用的有效方法[1-2]. 我们知道构成蛋白的基本单位是氨基酸,因此为了进一步探索磷酰化肽酯及小肽与蛋白相互作用的 机 理 , 采 用 ESI-MS 研 究 了 几 种 典 型 结 构 的 N- 二 异 丙 氧 基 磷 酰 化 肽 酯 及 小 肽 ( DPP ) ( 即 DIPP-L-Ala-Phe-OCH3 , DIPP-D-Ala-Phe-OCH3 , DIPP-L-Ala-Tyr-OCH3 , DIPP-Ala-Phe-OH , DIPP-Ala-Ala-OH)与20种蛋白氨基酸的相互作用,并考察了氨基酸的手性对相互作用的影响.
1 实验部分
1.1 主要仪器与试剂 纯水机Milli-Q (MA, USA)实验在Bruker-Esquire 3000电喷雾离子阱质谱仪 . (Bruker Daltonik Gmbh, Germany)上完成.所用的氨基酸均购自上海百泰生化公司;甲醇(色谱纯) :美国Fisher公司产品; N-磷酰化肽酯及小肽均参照文献方法合成[3],其结构均经过1H,13C,31P NMR和ESI-MSn确认. 1.2 质谱分析条件 喷雾电压 4 000 V,毛细管加热温度 200 ℃,锥孔电压135.1 V,喷雾气(N2)48.265 kPa,干燥 气 4.5 Lmin 1,导入离子源速率:4 Lmin 1(Cole-Parmer 744900 syringe pump),正离子检测模式.

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