SANTA CRUZ BIOTECHNOLOGY, INC. F-Spondin (N-19): sc-49006 Santa Cruz Biotechnology, Inc. 1.800.457.3801 831.457.3800 fax 831.457.3801 Europe +00800 4573 8000 49 6221 4503 0 www.scbt.com BACKGROUND F-Spondin, also designated Spondin-1 or vascular smooth muscle growth- promoting factor, is a member of the subgroup of the thrombospondin type 1 class molecules. F-Spondin is a secreted, extracellular matrix-attached protein which patterns axonal trajectories by promoting adhesion and outgrowth of commissural axons, in addition to inhibiting outgrowth of motor axons. F-Spondin contains two conserved domains at the amino terminus, FS1 and FS2, which are regions of homology with reelin and mindin. Additionally, F-Spondin contains either six or four thrombospondin repeats (TSRs) at the carboxyl terminus, which are typical of class 2 TSRs. The F-Spondin gene is expressed in the nervous system, mainly at the embryonic floor plate and the hippocampus. F-Spondin may play a role in promoting axonal regeneration after nerve injury and in inflammatory processes in the nervous system. REFERENCES 1. Burstyn-Cohen, T., Frumkin, A., Xu, Y.T., Scherer, S.S. and Klar, A. 1998. Accumulation of F-spondin in injured peripheral nerve promotes the out- growth of sensory axons. J. Neurosci. 18: 8875-8885. 2. Shimeld, S.M. 1998. Characterization of AmphiF-spondin reveals the modu- lar evolution of chordate F-spondin genes. Mol. Biol. Evol. 15: 1218-1223. 3. Tzarfaty-Majar, V., López-Alemany, R., Feinstein, Y., Gombau, L., Goldshmidt, O., Soriano, E., Mu?oz-Cánoves, P. and Klar, A. 2001. Plasmin-mediated release of the guidance molecule F-spondin from the extracellular matrix. J. Biol. Chem. 276: 28233-28241. 4. Tzarfati-Majar, V., Burstyn-Cohen, T. and Klar, A. 2001. F-spondin is a contact-repellent molecule for embryonic motor neurons. Proc. Natl. Acad. Sci. USA 98: 4722-4727. 5. Feinstein, Y. and Klar, A. 2004. The neuronal class 2 TSR proteins F-spondin and Mindin: a small family with divergent biological activities. Int. J. Biochem. Cell Biol. 36: 975-980. 6. Pyle-Chenault, R.A., Stolk, J.A., Molesh, D.A., Boyle-Harlan, D., McNeill, P.D., Repasky, E.A., Jiang, Z., Fanger, G.R. and Xu, J. 2005. VSGP/F-spondin: a new ovarian cancer marker. Tumour Biol. 26: 245-257. CHROMOSOMAL LOCATION Genetic locus: SPON1 (human) mapping to 11p15.2; Spon1 (mouse) mapping to 7 F1. SOURCE F-Spondin (N-19) is an affinity purified goat polyclonal antibody raised against a peptide mapping near the N-terminus of F-Spondin of human origin. PRODUCT Each vial contains 200 ?g IgG in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin. Blocking peptide available for competition studies, sc-49006 P, (100 ?g peptide in 0.5 ml PBS containing < 0.1% sodium azide and 0.2% BSA). APPLICATIONS F-Spondin (N-19) is recommended for detection of F-Spondin and mature Spondin-1 of mouse, rat and human origin by Western Blotting (starting dilution 1:200, dilution range 1:100-1:1000), immunofluorescence (starting dilution 1:50, dilution range 1:50-1:500) and solid phase ELISA (starting dilution 1:30, dilution range 1:30-1:3000). F-Spondin (N-19) is also recommended for detection of F-Spondin and mature Spondin-1 in additional species, including canine, bovine and avian. Suitable for use as control antibody for F-Spondin siRNA (h): sc-60613, F-Spondin siRNA (m): sc-60614, F-Spondin shRNA Plasmid (h): sc-60613-SH, F-Spondin shRNA Plasmid (m): sc-60614-SH, F-Spondin shRNA (h) Lentiviral Particles: sc-60613-V and F-Spondin shRNA (m) Lentiviral Particles: sc-60614-V. Molecular Weight of F-Spondin: 115 kDa. RECOMMENDED SECONDARY REAGENTS To ensure optimal results, the following support (secondary) reagents are recommended: 1) Western Blotting: use donkey anti-goat IgG-HRP: sc-2020 (dilution range: 1:2000-1:100,000) or Cruz Marker? compatible donkey anti-goat IgG-HRP: sc-2033 (dilution range: 1:2000-1:5000), Cruz Marker? Molecular Weight Standards: sc-2035, TBS Blotto A Blocking Reagent: sc-2333 and Western Blotting Luminol Reagent: sc-2048. 2) Immunofluo- rescence: use donkey anti-goat IgG-FITC: sc-2024 (dilution range: 1:100- 1:400) or donkey anti-goat IgG-TR: sc-2783 (dilution range: 1:100-1:400) with UltraCruz? Mounting Medium: sc-24941. STORAGE Store at 4° C, **DO NOT FREEZE**. Stable for one year from the date of shipment. Non-hazardous. No MSDS required. RESEARCH USE For research use only, not for use in diagnostic procedures. PROTOCOLS See our web site at www.scbt.com or our catalog for detailed protocols and support products.
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SANTA CRUZ BIOTECHNOLOGY, INC. F-Spondin (N-19): sc-49006 Santa Cruz Biotechnology, Inc. 1.800.457.3801 831.457.3800 fax 831.457.3801 Europe +00800 4573 8000 49 6221 4503 0 www.scbt.com BACKGROUND F-Spondin, also designated Spondin-1 or vascular smooth muscle growth- promoting factor, is a member of the subgroup of the thrombospondin type 1 class molecules. F-Spondin is a secreted, extracellular matrix-attached protein which patterns axonal trajectories by promoting adhesion and outgrowth of commissural axons, in addition to inhibiting outgrowth of motor axons. F-Spondin contains two conserved domains at the amino terminus, FS1 and FS2, which are regions of homology with reelin and mindin. Additionally, F-Spondin contains either six or four thrombospondin repeats (TSRs) at the carboxyl terminus, which are typical of class 2 TSRs. The F-Spondin gene is expressed in the nervous system, mainly at the embryonic floor plate and the hippocampus. F-Spondin may play a role in promoting axonal regeneration after nerve injury and in inflammatory processes in the nervous system. REFERENCES 1. Burstyn-Cohen, T., Frumkin, A., Xu, Y.T., Scherer, S.S. and Klar, A. 1998. Accumulation of F-spondin in injured peripheral nerve promotes the out- growth of sensory axons. J. Neurosci. 18: 8875-8885. 2. Shimeld, S.M. 1998. Characterization of AmphiF-spondin reveals the modu- lar evolution of chordate F-spondin genes. Mol. Biol. Evol. 15: 1218-1223. 3. Tzarfaty-Majar, V., López-Alemany, R., Feinstein, Y., Gombau, L., Goldshmidt, O., Soriano, E., Mu?oz-Cánoves, P. and Klar, A. 2001. Plasmin-mediated release of the guidance molecule F-spondin from the extracellular matrix. J. Biol. Chem. 276: 28233-28241. 4. Tzarfati-Majar, V., Burstyn-Cohen, T. and Klar, A. 2001. F-spondin is a contact-repellent molecule for embryonic motor neurons. Proc. Natl. Acad. Sci. USA 98: 4722-4727. 5. Feinstein, Y. and Klar, A. 2004. The neuronal class 2 TSR proteins F-spondin and Mindin: a small family with divergent biological activities. Int. J. Biochem. Cell Biol. 36: 975-980. 6. Pyle-Chenault, R.A., Stolk, J.A., Molesh, D.A., Boyle-Harlan, D., McNeill, P.D., Repasky, E.A., Jiang, Z., Fanger, G.R. and Xu, J. 2005. VSGP/F-spondin: a new ovarian cancer marker. Tumour Biol. 26: 245-257. CHROMOSOMAL LOCATION Genetic locus: SPON1 (human) mapping to 11p15.2; Spon1 (mouse) mapping to 7 F1. SOURCE F-Spondin (N-19) is an affinity purified goat polyclonal antibody raised against a peptide mapping near the N-terminus of F-Spondin of human origin. PRODUCT Each vial contains 200 ?g IgG in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin. Blocking peptide available for competition studies, sc-49006 P, (100 ?g peptide in 0.5 ml PBS containing < 0.1% sodium azide and 0.2% BSA). APPLICATIONS F-Spondin (N-19) is recommended for detection of F-Spondin and mature Spondin-1 of mouse, rat and human origin by Western Blotting (starting dilution 1:200, dilution range 1:100-1:1000), immunofluorescence (starting dilution 1:50, dilution range 1:50-1:500) and solid phase ELISA (starting dilution 1:30, dilution range 1:30-1:3000). F-Spondin (N-19) is also recommended for detection of F-Spondin and mature Spondin-1 in additional species, including canine, bovine and avian. Suitable for use as control antibody for F-Spondin siRNA (h): sc-60613, F-Spondin siRNA (m): sc-60614, F-Spondin shRNA Plasmid (h): sc-60613-SH, F-Spondin shRNA Plasmid (m): sc-60614-SH, F-Spondin shRNA (h) Lentiviral Particles: sc-60613-V and F-Spondin shRNA (m) Lentiviral Particles: sc-60614-V. Molecular Weight of F-Spondin: 115 kDa. RECOMMENDED SECONDARY REAGENTS To ensure optimal results, the following support (secondary) reagents are recommended: 1) Western Blotting: use donkey anti-goat IgG-HRP: sc-2020 (dilution range: 1:2000-1:100,000) or Cruz Marker? compatible donkey anti-goat IgG-HRP: sc-2033 (dilution range: 1:2000-1:5000), Cruz Marker? Molecular Weight Standards: sc-2035, TBS Blotto A Blocking Reagent: sc-2333 and Western Blotting Luminol Reagent: sc-2048. 2) Immunofluo- rescence: use donkey anti-goat IgG-FITC: sc-2024 (dilution range: 1:100- 1:400) or donkey anti-goat IgG-TR: sc-2783 (dilution range: 1:100-1:400) with UltraCruz? Mounting Medium: sc-24941. STORAGE Store at 4° C, **DO NOT FREEZE**. Stable for one year from the date of shipment. Non-hazardous. No MSDS required. RESEARCH USE For research use only, not for use in diagnostic procedures. PROTOCOLS See our web site at www.scbt.com or our catalog for detailed protocols and support products.