BD BioCoat Angiogenesis System:
Endothelial Cell Migration
Catalog Nos. 354143, 354144, 354147, 354148
Guidelines for Use
FOR RESEARCH USE ONLY
NOT FOR USE IN DIAGNOSTIC PROCEDURES
BD Biosciences Discovery Labware
Two Oak Park, Bedford, MA 01730, tel: 800.343.2035, fax: 800.743.6200, www.bdbiosciences.com
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SPC-354143-G rev 3.0
TABLE OF CONTENTS
Intended Use.3
Materials Provided.3
Materials Required But Not Supplied.4
Precautions.4
Procedure for Use.4
1.0 Equilibration of inserts to room temperature.5
2.0 Migration Studies: Post-Cell Migration Labeling with Calcein AM.5
3.0 Measurement of Cell Migration: Post-Cell Migration Labeling with Calcein AM.6
4.0 Migration Studies: Pre-Cell Migration Labeling.7
5.0 Measurement of Cell Migration: Pre-Cell Migration Labeling.7
6.0 Data Reduction.7
Guidelines for Automated Use 8
General Plate Reader Setup Details.9
Insert System Assembly & Orientation 9
Autofluorescence Background 9
Fluorescence Detection Issues.9
Stability.9
Quality Control 9
Customer and Technical Service 10
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SPC-354143-G rev 3.0
INTENDED USE
BD BioCoat Angiogenesis System: Endothelial Cell Migration provides endothelial cells with
conditions that allow assessment of their migratory property in vitro. By combining the
fluorescence blocking capabilities of the BD Falcon FluoroBlok membrane with the benefits
of an extracellular matrix, the process of screening for endothelial cell migration has been
optimized.
The BD BioCoat Angiogenesis System: Endothelial Cell Migration is a reliable, robust in vitro
assay system suitable for manual or robotic screening of pro- and anti-angiogenic compounds.
It is composed of a BD Falcon 24- or 96-Multiwell Insert Plate containing a fluorescence

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