et al. unpublished data). In order to evaluate the effect of
BFP on the cardiovascular system, we undertook the present
study to investigate its effect on blood coagulation and
platelet aggregation both in vitro and in vivo using prothrom-
bin time (PT), thrombin time (TT), activated partial thrombo-
plastin time (APTT), and platelet aggregation assays. The
same assays were also conducted to evaluate anticoagulant
and antiplatelet activities of the 26 ingredients from BFP.
MATERIALS AND METHODS
Animals ICR mice and New Zealand white rabbits were
obtained from the Laboratory Animal Service Center of the
Chinese University of Hong Kong. Animals were maintained
in an air-conditioned room with controlled temperature of
24 2 °C and humidity 55 15% in a 12 h light/dark cycle
regulation. The animals were allowed food and water ad libi-
tum. Animals were divided randomly into control and experi-
mental groups.
Reagents Collagen, adenosine diphosphate (ADP), and
archidonic acid (AA) reagents for platelet function testing
were obtained from Chrono-Log Co. (Havertown, PA,
U.S.A.). ThromborelR
S reagent, DadeR
thrombin time
reagent, and actin activated cephaloplastin reagent were pur-
chased from Bade Behring Narburg GmbH (Marburg, Ger-
many). Heparin sodium salt (from porcine intestinal mucosa,
187 USP units/mg), aspirin and indomethacin were obtained
from Sigma Chemical Co. (St. Louis, MO, U.S.A.). All drugs
were dissolved in Tris buffer (pH 7.4) contained 0.9%
sodium chloride. Other chemicals were of reagent grade and
were from commercial sources.
Extraction of BFP and Ingredients of BFP Five hun-
dred grams of Bak Foong Pills (obtained from Eu Yan Sang
Company, Hong Kong) was grinded and passed the 80
meshes. The powder (200 g) of BFP was reuxed using 70%
ethanol aqueous (1 l 3 times). The ltrate was concentrated

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