第33卷第1期 湖南农业大学学报(自然科学版) Vol.33 No.1
2007年2月 Journal of Hunan Agricultural University (Natural Sciences) Feb.2007
文章编号:1007-1032(2007)01-072-04
木霉菌对几种植物病原菌的拮抗作用
肖 烨1,易图永1*,魏 林2,李小娟1
(1.湖南农业大学 生物安全科技学院,湖南 长沙 410128; 2.湖南省植物保护研究所,湖南 长沙 410125)
摘 要:为探明木霉对植物病原菌的拮抗作用效果,筛选出优势菌株,从长沙地区的15种作物根围采集到27
份土壤样本,经室内分离,纯化得到28个木霉菌菌株.利用对峙法和稀释法测定了28个菌株对花生立枯病菌,
水稻纹枯病菌,水稻恶苗病菌,辣椒炭疽病菌,白术白绢病菌的拮抗作用,得到1株对5种供试病原真菌均有强
烈拮抗作用的菌株,经鉴定该菌株为哈茨木霉.对峙培养可观察到,多数情况下,接种后2 d内木霉与病原菌接
触,随后覆盖或侵入病菌菌落,抑制其生长,其抑制程度随木霉孢子浓度的降低而减弱.温室盆栽试验发现,哈
茨木霉对番茄立枯病防效显著,喷洒高浓度的哈茨木霉分生孢子悬浮液防效可达80%以上,且具有持续防效和刺
激作物生长的作用.
关键词:木霉菌;病原真菌;拮抗作用;番茄立枯病
中图分类号:S432.4 文献标识码:A
Study on antagonism of Trichoderma to fungal pathogens
XIAO Ye1,YI Tu-yong 1*,WEI Lin 2,LI Xiao-juan1
(1.College of Bio-Safety Science and Technology,HNAU,Changsha 410128,China;2.Hunan Plant Protection
Research Institute,Changsha 410125,China)
Abstract:In order to get the effect of mold control plant pathogeny,and find the best one,28 Trichoderma spp. strains
were isolated from 27 soil samples collected from the rhizosphere of 15 different crops around Changsha,their
antagonisms to Rhizoctonia solani,Rhizoctonia solani,Fusarium moniliforme,Collectotrichum capsici,Sclerotium
rolfsii were tested by dual culture and dilution.One strain could antagonize five tested fungal pathogens and was
identified to Trichoderma harziaum.The results in dual culture demonstrated that in most cases,Trichoderma spp. made
contact with the pathogens inoculation subsequently the pathogenic colonies were either overgrown or invaded by
Trichoderma spp.,leading to the inhibition in pathogen growth.The control extent along with spore concentration
debasement and reduction. Greenhouse pot experiment showed,the effect of Trichoderma harziaum control tomato
seeding wilt is very salience.Insufflation the high conidiophores suspend liquid of Trichoderma harziaum,the

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