CJCM 中华实用中西医杂志 2007年 VOL.(20) NO.4 -277-
·论著·
粉防己碱对缺血
灌注中TNF-α,IL-6表达的影响
The effect of tetrandrine on the expression
of TNF-α,IL-6 during myocardial ischemia/reperfusion
王裕勤 常 超 冯义柏 杨 刚 荣术玲 贺立群
(华中科技大学同济医学院附属协和医院,湖北 武汉,430022)
WANG Yu-qin Chang chao FENG Yi-bai et al
(Department of Cardiology,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology, Wuhan 430022,China)
中图分类号:R 743.31 文献标识码:A 文章编号:1607-2286(2007)04-0277-04 证型:BDG
【摘 要】目的:探讨心肌缺血/再灌注过程中粉防己碱(Tet)对肿瘤坏死因子-α(TNF-α),白细胞介素-6(IL-6)表达影响
及机制.方法:60只健康雄性SD大鼠随机分为3组:缺血/再灌注损伤组(I/R),假手术组(Sham),粉防己碱治疗组(Tet),I/R
组结扎大鼠冠状动脉左前降支造成心肌缺血30分钟后再灌注24小时后处死大鼠;Sham组只穿针不结扎,余步骤同I/R组;Tet
组在缺血前20分钟腹腔注射Tet,余步骤同I/R组.24小时后取心肌标本,用酶联免疫吸附法(ELISA)检测心肌组织中TNF-α,
IL-6表达水平,应用免疫组织化学方法检测磷酸化IκB-α(P-IκB-α)的表达,凝胶电泳迁移率(EMSA)变化检测NF-κB的活性.
结果:Tet组与I/R组相比TNF-α,IL-6表达水平明显减低(208.40±25.12 VS 306.65±17.78,P<0.01),(587.40±137.40 VS1003.75
±138.33,P<0.01)而与假手术组相比表达明显增强(208.40±25.12 VS 61.45±9.20,P<0.01),(587.40±137.40 VS 229.25±90.13,
P<0.01).Tet组大鼠心肌细胞胞浆中P-IκB-α的表达明显低于I/R 组(0.0817±0.09 vs 0.1755±0.01,P<0.01),但明显高于Sham
组(0.0817±0.09 vs 0.0513±0.03,P<0.01).Tet组NF-κB的活性明显低于I/R组(29.58±1.56 VS 40.33±4.39,P0.05).结论:Tet可以通过抑制IκB-α磷酸化有效抑制心肌缺血/再灌注时NF-κ
B的活化,减少TNF-α,IL-6的产生,起到减轻心肌缺血/再灌注损伤的作用.
【关键词】粉防己碱;缺血/再灌注;核因子-κB;肿瘤坏死因子-α;白细胞介素-6
【Abstract】Objective:The aim of this study is to investigate the effect and mechanism of tetrandrine(Tet) on the expression of TNF-
α,IL-6 during rat myocardial reperfusion period.Methods:60 male Sprague-Dawley rats were randomly divided into 3 groups:Sham group,
ischemia/reperfusion(I/R)group,Tet group.The Sprague-Dawley rat underwent 30 min of left anterior descending (LAD) coronary occlusion
and 24 hours reperfusion to make ischemia/reperfusion (I/R) injury model in vivo.Sham group were not subjected to occlusion of artery.Tet
group were injected tetrandrine to abdominal cavity 20 minute before ischemia starting.The other procedures were same to the ischemia/re-

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